Hoefer dyna quant 200 manual

Fluorometer for sale tractor parts and replacement. The dyna quant 200 quantitates dna on the basis of the fluorescent hoechst 33258 dyedoublestranded. Hoefer pharmacia biotech dyna quant 200 fluorometer. This fluorometer boots up, passes all self tests, responds to keypad input and zeros as i think it should. Phylogenetic placement of plant pathogenic sclerotium. Apoe genotypes associate with cognitive performance but not. Pharmacia biotech hoefer dyna quant 200 fluorometer model dq200unit powers on. The dye binds preferentially to doublestranded dna, thus allowing accurate quantitation. The dye binds preferentially to doublestranded dna. Hoefer dyna quant 200 fluorometer instructions hitachi model 10080 computerized spectrophotometer hp 5890 series ii gas chromatograph reference manual, site prepinstallation manual, and tutorial hp 5989a ms engine site preparation manual hp 7673 automatic sampler installing the hybrid injector bracket manual. Dna from pure cultures of azoxystrobinresistant alternaria isolates 37e3 and 37b8, and sensitive isolates a039 and 3a2, and from healthy pistachio leaves was also extracted by the fastdna kit and quantified using the hoefer dyna quant 200 fluorometer hoefer pharmacia biotech. Hoefer dyna quant 200 flourometer analytical instruments llc. The fusion plasmids integrated onto pqbr103 were recovered by a modified method of conjugative cloning 7,19.

Two cytokinin receptors of arabidopsis thaliana, cre1ahk4. Used hoefer dyna quant 200 fluorometer lab general for. Development of a qrtpcr assay to determine the relative mrna. Dna was quantified using a hoefer dyna quant 200 flurometer amersham biosciences, piscataway, nj, usa. Dec 01, 2004 the product, 7hydroxy4methylcoumarin 4mu was detected using the hoefer dyna quant 200 fluorometer amersham pharmacia biotech, san francisco, usa and the reaction was monitored at 460 nm with 365 nm excitation wavelength. Measurement of dna concentrations using hoechst 33258. A realtime pcr assay for the detection of azoxystrobin. Dyna quant 200 designed specifically for the accurate quantitation of low dna concentrations even in presence of arn, proteins andor nucleotides can also measure enzyme activity based on the cleavage of methylumbelliferyllinked substrates as well as. A realtime pcr assay for the detection of azoxystrobinresistant alternaria populations from pistachio orchards in. Measurement of nucleic acid concentrations using the dyna quant tm and the genequanttm. However, we test and provide details about many items we sell. Hoefer dyna quant 200 fluorometer fluorimeter hoefer dyna. Microplate fluorescence assay for the quantification of double stranded dna using sybr green i dye.

The nuclear pellet was resuspended in 5 volumes buffer d 25 mm hepes ph 7. Amersham hoefer dq200 dyna quant 200 dna dynaquant fluorometer. The hoefer dyna quant 200 fluorometer, model dq200115v, is designed specifically for the accurate quantitation of small volume samples with low dna concentrations using the hoechst 33258 dye. For the best chance of winning, increase your maximum bid. We have evaluated single nucleotide polymorphisms snps in the fbxo11 gene for association with chronic otitis media with effusionrecurrent. Quantstudio 3 and 5 realtime pcr systems installation, use, and maintenance firmware v1. Genomic dna was extracted from white blood cells using the standard phenolchloroform method. And the dyna quant 200 sensitivity is 200300 times greater than uvvis methods. The dye binds preferentially to doublestranded dna, thus allowing accurate quantitation even in the presence of rna, proteins. Big savings on 200 excellent, buy now at deep discounts. The dyna quant 200 fluorometer is just a fraction of the cost of a uvvisible spectrophotometer. Microplate fluorescence assay for the quantification of. After isolation, the concentration of the plasmid cdna was determined in a hoefer dyna quant 200 fluorometer amersham pharmacia.

Find online auctions and classified ads for scientific equipment on labx. Simultaneously, quantification of dog hair samples 10, 30, 50 and 100 single dog hairs, 15 mm length was performed spectrophotometrically using hoefer dyna quant 200 fluorometer. Our stateoftheart testing facility in tulsa, oklahoma has tested and verified every aspect of this reel in conditions above and beyond what you may. Fluorescence was measured using a hoefer dyna quant 200 amersham biosciences, san francisco, u. The dna concentration was determined using a dyna quant 200 fluorometer hoefer. The intermediate layer where white blood cells were concentrated was collected and resuspended in phosphatebuffered saline pbs for further processing for dna analysis. Samples mixed with sybr green i in the wells of a microtiter plate produced fluorescence in proportion with dna concentration which was measured using a fluorescence plate reader. Thank you for choosing quantum your new quantum reel has been designed, engineered and built to be the best in the world. A highthroughput, 96well microplate fluorescence assay mfa was developed for dna quantification using the doublestranded dnabinding dye sybr green i. Objective the fbxo11 gene is the human homologue of the gene mutated in the novel deaf mouse mutant jeff jf, a single gene model of otitis media. Dna quantities were converted to cell numbers by using a conversion factor of 8 pg of dna per cell 16. Designed specifically for the accurate quantitation of small volume samples with low dna.

Total genomic dna was purified from wholeblood samples obtained from mind participants using the puregene dna isolation kit gentra, inc. Statistical analysis was performed by paired onetailed students t test. Laboratory equipment manuals hitechtrader used lab equipment. Only accessories and parts approved or supplied by hoefer, inc. Singer futura ce200 manuals manuals and user guides for singer futura ce200. This instrument is designed for indoor laboratory use only. Sep 15, 2004 fluorescence was measured using a hoefer dyna quant 200 amersham biosciences, san francisco, u. Measurement of dna concentrations using hoechst 33258 dye in the hoefer dyna quant flourometer. Hoefer dyna quant 200 fluorometer, model dq200115v, is designed specifically for the accurate quantitation of small volume samples with low dna c.

List names where possible all occupants of lab g10 and users of the hoefer dyna quant 200 fluorometer 3. Novel approach for assessing performance of pcr cyclers. Association of the fbxo11 gene with chronic otitis media. Purified genomic dna was digested with the same restriction enzyme as that used for generation of the crosslinked template, and.

Used hoefer dyna quant 200 fluorometer dna related for. Labtrader is becoming premier biotech equipment same great equipment, service, and staff. Model tko 100 hoefer tko 100 dna fluorometer tko 100 hoefer scientific instruments dna fluorometer model tko 100 hoefer tko100 dna fluorometer used nice. Amersham hoefer dq200 dyna quant 200 dna dynaquant. To find the total mass of the plasmid cdna, the mass of a. You need to be registered and approved to bid at this auction. Attach copies of data sheets name amount hazard risk phrases hazdat no biosciencesno hoechst. The od 600 of the remaining culture was determined and the. Weve been doing this for over 25 years and repair all that we sell.

Dq200 dq manual with fluorometer hoefer 200 dq with hoefer dq200 200 manual fluorometer hoefer dq. Fluorometer for sale pool, patio, and bbq supplies. Samples were loaded at four corner positions a1, a12, h1, and h12 and one central position d4. May 23, 2007 dna from blood and buccal samples were extracted using qiagen qiamp dna blood midi and mini extraction kits qiagen, hilden, germany following the suggested protocols of the manufacturer. As control, samples with known dna concentrations were incubated with the same buffer. The performance characteris the assay of test samples, 2ll sample were diluted tics of the mfa were compared to spectropho 123 biotechnol lett 2006 28. Validation of a short tandem repeat multiplex typing. Automated sequencing applied biosystems 3730xl dna analyzer was performed at the iowa state university dna sequencing and synthesis facility. Small size fast analysis superior results at a low cost. We supply a wide range of brand new equipment for the laboratory ph, petroleum product testers, freezers, furnaces, microscopes and more.

Validation of a short tandem repeat multiplex typing system. Hoefer dyna quant 200 fluorometer excellent condition with manual. Dna concentration was determined using a hoefer dyna quant 200 fluorometer. Pharmacia biotech, san francisco, ca, usa was developed for the quantification of dna with the h33258 fluorochrome. Hoefer pharmacia biotech dyna quant 200 fluorometer ebay. Laboratory equipment manuals hitechtrader used lab. Measurement of nucleic acid concentrations using the dyna. Association of the fbxo11 gene with chronic otitis. Nuclei were then isolated by centrifugation for 30 seconds at 10,000 g. The hoefer dyna quant 200 fluorometer is designed specifically for measurement of low dna concentrations using the hoechst 33258 dye in low volume samples.

Dna concentration was quantified using standardized fluorometric readings on a hoefer dyna quant 200 fluorometer hoefer pharmacia biotech inc. California and quantified on a hoefer dyna quant 200 fluorometer amersham pharmacia biotech, san francisco, california. It is designed specifically for the accurate quantitation of small volume samples with low dna concentrations using hoechst 33258 dye. Ssllc offers a wide selection of used electrophoresis for your lab. Measurement of dna concentrations using hoechst 33258 dye in. Hoefer he100 he 100 supersub electrophoresis manual. The ge healthcare amersham biosciences dyna quant 200 dq 200 series fluorometers are designed specifically for the accurate quantitation of small volume samples with low dna concentrations using the hoechst 33258 dye. The instrument has a narrowband excitation light source.

Fluorometer function and description the hoefer dq 200 fluorometer is a. Evaluation of developmental phenotypes produced by morpholino. Quantstudio 6 and 7 flex realtime pcr systems maintenance and administration guide 11 about this guide purpose this user guide provides provides re ference information for the quantstudio 6 and 7 flex realtime pcr systems and describes how to prepare, maintain, and troubleshoot the systems. Dyna quant 200 fluorometer is designed specifically for the accurate quantitation of small volume samples with. The mass of a single plasmid cdna copy was calculated by the formula. Universal resource trading ltd used lab equipment dealer on. The dyna quant 200 fluorometer is designed specifically for the accurate quantitation of small volume samples with low dna concentrations using the hoechst 33258 dye. Sep 10, 20 the dyna quant 200 fluorometer is designed specifically for the accurate quantitation of small volume samples with low dna concentrations using the hoechst 33258 dye. Seller assumes all responsibility for this listing. We have 3 singer futura ce200 manuals available for free pdf download. Analytical instruments is a trusted source for new and used lab equipment and lab instruments for those establishing new laboratories or expanding existing laboratories. Hoefer tko100 dna fluorometer quantitative analysis. Find hitachi, thermo fisher scientific, and shimadzu for sale on machinio.